Regulatory

Part:BBa_K2983051:Design

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)


pTef1d: wild-type without the BsaI site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotides 1227374 to 1226969 of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1) with the BsaI site (GGTCTC) changed to GGTCTg.


Source

DNA synthesis

References